张可伟,教授,博士生导师。2006年获中科院遗传发育所博士学位;2006-2009在美国康奈尔大学进行博士后研究;2009-2013年在美国布鲁克海文国家实验室任研究助理;2013年底至今任浙江师范大学教授。近年以通讯作者或第一作者在Nature Communi、PNAS、 Plant Cell等国内外高水平学术杂志上发表论文30多篇,获国家发明专利3项,美国发明专利2项。研究成果多次被Faculty 1000杂志重点评述推荐,并被Science Daily等新闻媒体报道。现任中国植物生理与植物分子生物学会理事、植物衰老与后熟专业委员会委员、浙江师范大学学术委员会委员。 担任Molecular Plant, Plant Cell, New Phytologist等国际期刊审稿人。
研究方向:植物生长发育(衰老)的激素调控机理研究与应用
以模式植物拟南芥和农作物水稻为实验材料,研究激素合成、运输和信号转导在植物生长发育(衰老)过程中的作用机理,揭示重要农艺性状的分子调控机理,探索作物改良的新途径。主要包括以下三方面的工作:
1. 植物(叶片)衰老调控机理研究:植物衰老是基本的生命现象,与作物产量、品质以及抗逆密切相关。植物衰老受多种激素的协同调控,其中细胞分裂素起正调控作用,而水杨酸起负调控作用。我们拟通过研究这两种激素调控植物衰老的信号途径,深入了解激素调控植物衰老的分子机理。同时通过正向遗传学克隆控制水稻叶片衰老的关键基因,鉴定在品种之间存在功能多样性的等位基因,获得能用于品种改良的种质资源。
2. 植物激素运输机制研究:植物激素的合成和发挥功能的位置往往不同。激素在合成后,需要经过复杂的运输途径将激素从合成部位运输到与受体结合部位。我们的研究兴趣在于鉴定细胞分裂素、水杨酸、脱落酸等激素的转运蛋白及其运输途径,阐明其在植物生长发育中的作用机理。
3. 植物激素代谢与调控机制研究:植物激素是一种微量的次生代谢产物,激素的合成和降解(失活)受到精确调控,同时也是植物调控激素信号途径的一种重要方式。水杨酸合成主要有两种途径:ICS途径和PAL途径,其中ICS途径已经被阐明清楚,PAL 途径尚不清楚。水稻是水杨酸含量最高的植物之一,我们以水稻和拟南芥为研究材料研究水杨酸的代谢和分解的生化路径和调控,阐明其在生长发育和抗病中的调控机理。
代表性科研项目:
1. 浙江省自然科学基金重点项目:LZ23C020001、氮信号通过水杨酸途径调控叶片衰老的分子机理研究
2. 国家重点研发计划育种专项子课题:2016YFD010091、细胞分裂素调控水稻产量的分子机理和应用
3.国家自然科学基金面上项目:31670277、水杨酸羟基化调控水杨酸动态平衡的分子机理
4.国家自然科学基金面上项目:31470370、拟南芥ABCG14细胞分裂素转运复合体的鉴定和作用机理研究
5.浙江省杰出青年基金:LR15C020001、水杨酸5-羟基化酶S5H的鉴定及其在叶片衰老和抗病过程中的功能研究
6.浙江师范大学高层次人才引进专项经费:植物衰老的激素调控机理研究
代表性论文:
Huang, P., Zhao, J., Hong, J., Zhu, B., Xia, S., Zhu, E., Han, P., and Zhang, K*. (2023). Cytokinins regulate rice lamina joint development and leaf angle. Plant physiology 191, 56-69.
Zhao, J., Deng, X., Qian, J., Liu, T., Ju, M., Li, J., Yang, Q., Zhu, X., Li, W., Liu, C.J., Jin, Z., and Zhang, K*. (2022). Arabidopsis ABCG14 forms a homodimeric transporter for multiple cytokinins and mediates long-distance transport of isopentenyladenine-type cytokinins. Plant communications, 100468.
Zhang, Y., Yu, Q., Gao, S., Yu, N., Zhao, L., Wang, J., Zhao, J., Huang, P., Yao, L., Wang, M*., and Zhang, K*. (2022). Disruption of the primary salicylic acid hydroxylases in rice enhances broad-spectrum resistance against pathogens. Plant, cell & environment 45, 2211-2225.
Cai, Z., Guo, H., Shen, S., Yu, Q., Wang, J., Zhu, E., Zhang, P., Song, L., Zhang, Y.*, and Zhang, K*. (2022). Generation of the salicylic acid deficient Arabidopsis via a synthetic salicylic acid hydroxylase expression cassette. Plant methods 18, 89.
Guo, Y#., Ren, G#., Zhang, K.#, Li, Z.#, Miao, Y.*, and Guo, H.* (2021). Leaf senescence: progression, regulation, and application. Molecular Horticulture 1:5
Zhao, J., Ding, B., Zhu, E., Deng, X., Zhang, M., Zhang, P., Wang, L., Dai, Y., Xiao, S., Zhang, C., Liu, C.J., and Zhang, K. (2021). Phloem unloading via the apoplastic pathway is essential for shoot distribution of root-synthesized cytokinins. Plant physiology 186, 2111-2123. (Faculty 1000 推荐https://facultyopinions.com/article/740013531)
Jiangzhe Zhao, Min Ju, Jiayun Qian, Mengyuan Zhang, Ting Liu, Kewei Zhang*. A tobacco syringe agroinfiltration-based method for a phytohormone transporter activity assay using endogenous substrates, Frontiers in Plant Science, 2021, 12: 660966.
Wei Zhang#, Kaixuan Peng#, Fubin Cui#, Dongling Wang, Jiangzhe, Zhao, Yanjun, Zhang, Ningning, Yu, Yuyang, Wang, Dali Zeng, Yonghong Wang, Zhukuan, Cheng, Kewei Zhang*. Cytokinin oxidase/dehydrogenase OsCKX11 coordinates source and sink relationship in rice by simultaneous regulation of leaf senescence and grain number. Plant Biotechnology Journal, 2021, 19(2):335-350.
Mengyuan Zhang, Bingli Ding, Jiangzhe Zhao, Penghong Zhang, Yujia Li, Guodong Yang, Kewei Zhang*. A fluorescence-based high-throughput screening method for cytokinin translocation mutants,Plant Methods,2020, 16:134
Jiangzhe Zhao, Ningning Yu, Min Ju, Biao Fan, Yanjun Zhang, Engao Zhu, Mengyuan Zhang, and Kewei Zhang*. ABC transporter OsABCG18 controls the shootward transport of cytokinins and grain yield in rice. Journal of Experimental Botany,2019,70, 6277-6291.
Zhang, Y#; Zhao, L#; Zhao, J; Li, Y; Wang, J; Guo, R; Gan, S; Liu, C; Zhang, K*S5H/DMR6 encodes a salicylic acid 5-hydroxylase that fine-tunes salicylic acid homeostasis, Plant Physiology, 2017.11, 175(3): 1082-1093
Cai Y§, Zhang K§, Kim H, Hou G, Zhang X, Yang H, Feng H, Miller L, Ralph J, Liu CJ (2016) Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase.Nature Commun,2016,7: 11989 (§共同第一作者)
Zhang, K., Novak, O., Wei, Z., Gou, M., Zhang, X.,Yu, Y., Yang, H., Cai, Y., Strnad, M., and Liu C-J*.Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins. Nature Communi,2014, 10.1038/ncomms4274. Faculty of 1000推荐(http://f1000.com/prime/718272723)
Zhang, K.,Halitschke, R., Yin, C., Liu,C-J, Gan, S*. Salicylic acid 3-hydroxylase regulates Arabidopsis leaf longevity by mediating SA catabolism. PNAS,2013, 110 : 14807–14812.Faculty of 1000推荐(http://f1000.com/prime/718077385)
Zhang, K§.,Bhuiya, M.-M§., Pazo, J., Miao, Y., Kim, H., Ralph, J. and Liu, C.-J.*.An engineered monolignol 4-O-methyltransferase depresses lignin biosynthesis and confers novel metabolic capability in Arabidopsis. Plant Cell, 2012, 24: 3135-3152.
Zhang, K.,and Gan, S*.An abscisic acid-AtNAP transcription factor-SAG113 protein phosphatase 2C regulatory chain for controlling dehydration in senescing Arabidopsis leaves. Plant Physiol, 2012, 158: 961-969.
Zhang, K., Xia, X., Zhang, Y. and Gan, S*.An ABA-regulated and Golgi-localized protein phosphatase controls water loss during leaf senescence in Arabidopsis. Plant J, 2012, 69: 667-678.
Zhang, K§., Qian, Q§., Huang, Z., Wang, Y., Li, M., Hong, L., Zheng, D., Gu, M., Chu,C., and Cheng, Z.* GOLD HULL AND INTERNODE2 Encodes a Primarily Multifunctional Cinnamyl-Alcohol Dehydrogenase in Rice. Plant Physiol, 2006, 140: 972-983.
本课题组是处于上升期的植物激素与衰老研究团队,研究课题前沿,学习机会多,学术氛围浓厚、仪器设备先进、研究经费充足。热忱欢迎热爱科研的有志青年学生加入团队,共同探索植物科学的奥秘!联系方式:kwzhang@zjnu.edu.cn ; 电话:0579-82290711